Sec 1 to Sec 2
$20 (Inclusive of GST)
1 day

9.30am to 4.30pm (includes lunch break; food not provided)

With an increasing population of people with diabetes, there is a higher demand for insulin. To meet the increasing demand, we manufacture human insulin in the lab. Ever wondered how human insulin is produced in the lab? This is done by genetically engineering bacteria to produce insulin. Since its innovation in 1973, genetic engineering has been applied in many fields such as producing genetically modified food, genetic treatments, and medicines. Come and learn more about diabetes as well as the basics of genetic engineering. 

Objectives: 

1. Learn how genetic engineering can be used to treat genetic diseases such as diabetes. 
2. Learn how diabetes is being screened and diagnosed. 
3. Learn micropipetting techniques. 
4. Understand what happens during restriction digest and ligation through a paper activity. 
5. Understand how DNA is analysed through a technique known as agarose gel electrophoresis. 
6. Conduct a bacteria transformation experiment using the heat shock method.  
7. Learn more about the applications of genetic engineering. 

* Prior knowledge of micropipetting techniques is encouraged but not necessary.

Sec 3 to Sec 5
$30 (Inclusive of GST)
1.5 days

Day 1: 9.30am to 4.30pm / Day 2: 9.30am to 12.30pm (Includes lunch break; food not provided)

Have you wondered how human insulin is produced in the lab? This is done by genetically engineering bacteria to produce insulin. Since its innovation in 1973, genetic engineering has been applied in many fields such as producing genetically modified food, genetic treatments and medicines. Come and have a first-hand experience of conducting these authentic biotechnological experiments used by researchers around the world!

Objectives:

1. Define genetic engineering and recombinant DNA cloning. 
2. Learn micropipetting techniques. 
3. Perform an experiment on recombinant DNA cloning including digestion of DNA with restriction enzyme, ligation of desired vectors, bacteria transformation, selection for transformed bacteria, agarose gel electrophoresis^#, and purification of protein product using column chromatography^#. 
4. Learn more about the applications of genetic engineering. 
5. Explore different perspectives on the effect of genetic engineering. 

* Also available as a 1-day workshop upon request. Items with ^# will not be covered for 1-day workshop.

ABE Express: Genetic Engineering Advanced

JC1–JC2/tertiary level
$35 (Inclusive of GST)
2 days (Both days are not consecutive)

Day 1 & Day 2: 9.30am to 4.30pm (includes lunch break; food not provided)

Have you wondered how human insulin is produced in the lab? This is done by genetically engineering bacteria to produce insulin. Since its innovation in 1973, genetic engineering has been applied in many fields such as producing genetically modified food, genetic treatments, and medicines. Come and have a first-hand experience of conducting these authentic biotechnological experiments used by researchers around the world!

Objectives: 

1. Define genetic engineering and recombinant DNA cloning. 
2. Learn micropipetting techniques. 
3. Perform an experiment on recombinant DNA cloning including digestion of DNA with restriction enzyme, ligation of desired vectors, bacteria transformation, selection for transformed bacteria, agarose gel electrophoresis^#, purification of protein product using column chromatography^#, and colony polymerase chain reaction^#.
4. Learn more about the applications of genetic engineering. 
5. Explore different perspectives on the effect of genetic engineering. 

* Also available as a 1-day workshop upon request. Items with ^# will not be covered for 1-day workshop.