Colony PCR files from resource content

RI site edits

This is not part of the Amgen Biotech Experience labs. This is a lab restricted to those in the ABE RI program who have been previously trained on this exercise.

This PDF file contains all of the files pertaining to Lab 1.

This video reviews key content for Lab 2/2A: Preparing to Clone a Gene, including the concepts of restriction enzymes and recombinant plasmids. It goes on to explain how these concepts are put into practice during Lab 2 when students use restriction enzymes to begin to engineer a plasmid, as well as several tips and tricks such as minimizing the number of freeze-thaw cycles for your reagents.

This PDF file contains all of the files pertaining to Lab 2A.

This PDF file contains all of the files pertaining to Lab 4A.

This video contains an overview of ABE's Lab 5/5A: "Transforming Bacteria with the Ligation Products". Once a recombinant plasmid that includes the gene of interest has been created, the next step is to replicate the plasmid and allow the bacteria to produce the protein. 

In this lab, students carry out the transformation of E. coli bacteria using a recombinant plasmid that contains the rfp gene. If transformation was successful, bacteria will glow red because they are producing the red fluorescent protein.