Resources

Browse the resources below.

Resources
Resource File/s

Colony PCR files from resource content

Student Guide
Teacher Guide

RI site edits

Resource File/s

This is not part of the Amgen Biotech Experience labs. This is a lab restricted to those in the ABE RI program who have been previously trained on this exercise.

Resource File/s

This PDF file contains all of the files pertaining to Lab 1.

Video

This video reviews key content for Lab 2/2A: Preparing to Clone a Gene, including the concepts of restriction enzymes and recombinant plasmids. It goes on to explain how these concepts are put into practice during Lab 2 when students use restriction enzymes to begin to engineer a plasmid, as well as several tips and tricks such as minimizing the number of freeze-thaw cycles for your reagents.

Flow Chart
Resource File/s

This PDF file contains all of the files pertaining to Lab 2A.

Flow Chart
Resource File/s

This PDF file contains all of the files pertaining to Lab 4A.

Video

This video contains an overview of ABE's Lab 5/5A: "Transforming Bacteria with the Ligation Products". Once a recombinant plasmid that includes the gene of interest has been created, the next step is to replicate the plasmid and allow the bacteria to produce the protein. 

In this lab, students carry out the transformation of E. coli bacteria using a recombinant plasmid that contains the rfp gene. If transformation was successful, bacteria will glow red because they are producing the red fluorescent protein.