Foundations - Aliquoting Guide for Lab 2a
Foundations - Aliquoting Guide for Lab 2a
We have provided sufficient reagents for the number of students you indicated on the Materials Request form, including a bit extra for pipetting errors. All solutions should be aliquoted into the provided 1.5 mL microcentrifuge tubes, unless otherwise indicated.
| Label tube | Contents of tube | Aliquot | Actually used |
|---|---|---|---|
| RP-2a | pARA-R (70ng/µL) | 10µL | 8µL |
| RE | BamH I and Hind III | 3µL | 2µL |
| 2.5xB | 2.5x restriction buffer | 10µL | 8µL |
| *dH2O | Distilled water | 1000µL | 2µL |
*dH2O is used for several labs.
ABE Lab Specific Resources
Related Lab
Foundations - Lab 2A: Preparing to Clone a Gene