Aliquoting Guide for Lab 5a

We have provided sufficient reagents for the number of students you indicated on the Materials Request form, including a bit extra for pipetting errors. All solutions should be aliquoted into the provided 1.5 mL microcentrifuge tubes, unless otherwise indicated.

***We recommend having students bring their empty tubes on ice to you to receive the competent cells directly into their working tubes (50µL each to P- and P+) instead of aliquoting them before class. The cells perform best if they are used immediately after they are thawed.