Foundations - Aliquoting Guide for Lab 5

We have provided sufficient reagents for the number of students you indicated on the Materials Request form, including a bit extra for pipetting errors. All solutions should be aliquoted into the provided 1.5 mL microcentrifuge tubes, unless otherwise indicated.

Students will use the “Ligase” tube that was prepared in Lab 3; it contains 10 µL of their recombinant plasmid and you will not have to label or aliquot these.

***We recommend having students bring their empty tubes on ice to you to receive the competent cells directly into their working tubes (50µL each to P- and P+) instead of aliquoting them before class. The cells perform best if they are used immediately after they are thawed.