Foundations - Aliquoting Guide for Lab 4a
Foundations - Aliquoting Guide for Lab 4a
We have provided sufficient reagents for the number of students you indicated on the Materials Request form, including a bit extra for pipetting errors. All solutions should be aliquoted into the provided 1.5 mL microcentrifuge tubes, unless otherwise indicated.
Label tube | Contents of tube | Aliquot | Actually used |
---|---|---|---|
M | 1 kb ladder | 10µL | 10µL |
LD | Loading dye | 6µL | 4µL |
Reminder: The ladder already contains loading dye. Also, Solution 2 (Lab 1) is the same as loading dye.
ABE Lab Specific Resources
Related Lab
Foundations - Lab 4A: Verifying the Recombinant Plasmid