Foundations - Aliquoting Guide for Lab 4
Foundations - Aliquoting Guide for Lab 4
We have provided sufficient reagents for the number of students you indicated on the Materials Request form, including a bit extra for pipetting errors. All solutions should be aliquoted into the provided 1.5 mL microcentrifuge tubes, unless otherwise indicated.
| Label tube | Contents of tube | Aliquot | Actually used |
|---|---|---|---|
| M | 1 kb ladder | 10µL | 10µL |
| LD | Loading dye | 12µL | 10µL |
| *dH2O | Distilled water | 1000µL | 19µL |
*dH2O is used for several labs.
Reminder: The ladder already contains loading dye. Also, Solution 2 (Lab 1) is the same as loading dye.
ABE Lab Specific Resources
Related Lab
Foundations - Lab 4: Verifying the Recombinant Plasmid