Foundations - Aliquoting Guide for Lab 3
Foundations - Aliquoting Guide for Lab 3
We have provided sufficient reagents for the number of students you indicated on the Materials Request form, including a bit extra for pipetting errors. All solutions should be aliquoted into the provided 1.5 mL microcentrifuge tubes, unless otherwise indicated.
| Label tube | Contents of tube | Aliquot | Actually used |
|---|---|---|---|
| 5xB | 5x ligation buffer | 4µL | 3µL |
| **LIG | T4 DNA ligase | 2µL | 2µL |
| *dH2O | Distilled water | 1000µL | 2µL |
**NOTE: Students work directly in the LIG tube, do not aliquot any extra.
*dH2O is used for several labs.
ABE Lab Specific Resources
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Foundations - Lab 3: Building a Recombinant Plasmid