Foundations - Aliquoting Guide for Lab 2
Foundations - Aliquoting Guide for Lab 2
We have provided sufficient reagents for the number of students you indicated on the Materials Request form, including a bit extra for pipetting errors. All solutions should be aliquoted into the provided 1.5 mL microcentrifuge tubes, unless otherwise indicated.
Label tube | Contents of tube | Aliquot | Actually used |
---|---|---|---|
A | pARA (80ng/µL) | 10µL | 8µL |
pKAN-R (80ng/µL) | 10µL | 8µL | |
RE | BamH I and Hind III | 5µL | 4µL |
2.5xB | 2.5x restriction buffer | 20µL | 16µL |
*dH2O | Distilled water | 1000µL | 4µL |
*dH2O is used for several labs.
ABE Lab Specific Resources
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Foundations - Lab 2: Preparing to Clone a Gene